Bayer School of Natural and Environmental Sciences
Peter A. Castric
Kyle W. Selcer
Glycosylation, Gram-negative Bacteria, Microbial Physiology, Pathogenesis
The pili of P. aeruginosa 1244 are involved in a post-translational modification in which an acidic carbohydrate moiety structurally similar to the lipopolysaccahride (LPS) O-antigen is covalently attached to the pilin protein. It has been shown that this associated glycan is evenly distributed over the entire surface of the pilus. Because of its prime location along the pilus, experiments were conducted to determine if the pilin glycan contributed to the virulence of P. aeruginosa. Initial studies involved the assessment of LD50 values to demonstrate that acquisition of the exoU gene increased the virulence of PA1244. Interestingly, the LD50 studies suggested a role for the pilin glycan in PA1244 virulence. As a more sensitive determination of virulence, a competition assay utilizing a murine model of acute pneumonia demonstrated increased virulence associated with the glycan. Furthermore, employment of a nonopsonic phagocytosis assay has indicated inhibition of phagocytosis correlated with the pilin glycan.
Roguskie, J. (2005). The Role of the Pseudomonas aeruginosa 1244 Pilin Glycan in Virulence (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/1119