Bayer School of Natural and Environmental Sciences
Joseph R. McCormick
Aerial Filaments, Cell Division, C-terminal Core Domain, FtsZ, Intragenic Suppression, Streptomyces coelicolor
FtsZ is the earliest known acting protein in the prokaryotic division machine. In Streptomyces coelicolor, mutations in ftsZ result in hyphae that cannot support division and the formation of uninucleoid spores. Part of this study focused on isolating intragenic suppressors to the ftsZ(A275V) mutation to investigate the function of the C-Terminal Domain of the protein. To date, eight intragenic suppressors of A275V have been isolated, three in this study. Their distribution in FtsZ is scattered, although there seems to be a preference for mutations that are near the GTP-binding domain. Also, the function of the highly conserved C-Terminal Core Domain of FtsZ was investigated using a similar strategy, but no intragenic suppressor mutations were isolated. Finally, a development-specific promoter tool was created to allow the expression of an ftsZ allele in aerial filaments alone. One can now express any ftsZ allele to visualize the division phenotype during sporulation septation.
Szabo, A. (2006). The Isolation of Intragenic Suppressors to Point Mutations In the 3' Coding Region of ftsZ of Streptomyces coelicolor (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/1263