Defense Date
11-7-2023
Graduation Date
Fall 12-15-2023
Availability
One-year Embargo
Submission Type
thesis
Degree Name
MS
Department
Biomedical Engineering
School
School of Science and Engineering
Committee Chair
Bin Yang
Committee Member
Kimberly Williams
Committee Member
Kyle Selcer
Committee Member
Ipsita Banerjee
Keywords
Organoid, Pluripotent Stem Cell, Alginate, Air-jetting Bioprinting, Cell Encapsulation, Type-1 Diabetes, 3D Stem Cell Culture, Tissue Engineering, Droplet based Bioprinting, Hydrogel, Coaxial Air Channel
Abstract
Biomanufactured 3D-organoids show promise for modeling and treating various conditions, particularly insulin-dependent diabetes mellitus (type-1 diabetes). Pancreatic islet organoids serve as sustainable sources of insulin-producing β-cells, potentially restoring insulin regulation in patients and providing a long-term solution for diabetes. Hydrogel droplet printing is an emerging technique in regenerative medicine due to its advantageous tunability and biocompatibility; however, challenges of consistency, quality control, and scalability persist. To address these issues, we developed a novel air-jetting based droplet bioprinting system for the generation of monodispersed alginate micro-droplets. The objective of this project was to incorporate human induced pluripotent stem cells (hiPSCs) into these micro-droplets while maintaining uniformity, size, viability, and functionality. Comprehensive parametric studies led to the standardization of our process for the scalable production of spherical stem cell-laden micro-droplets within a customizable size range (300-650μm). Ongoing investigations into cell seeding density, aggregation, and pluripotency will enhance our role of cell encapsulation in the organoid generation pipeline. Herein, we aim to improve the scalability and quality control of bioprinting stem cell-laden micro-droplets to facilitate the manufacturing of suitable organoids for islet transplantation.
Language
English
Recommended Citation
Stump, M. B. (2023). Air-Jet Bioprinting of Stem Cell-Encapsulated Alginate Micro-Droplets for Organoid Biomanufacturing (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/2293
Included in
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