Chemistry and Biochemistry
Bayer School of Natural and Environmental Sciences
bioactive lipids, fatty amides, HPLC, mass spectrometry
Bioactive lipids are known to exert physiological effects and interact with neuroreceptors. Little is known about the bioregulation of primary fatty acid amides, though N-acyl glycines are thought to be their anabolic precursors. Chapter 1 details the current metabolic, physiological, and receptor interactions of primary fatty acid amides and their related congeners and reviews current strategies for isolation and detection. Chapter 2 outlines mass spectrometry collision induced fragmentation assignments pertaining to method development of multiple reaction monitoring detection of these species. Chromatographic separation methods of fatty acyls, with a focus on primary fatty acid amides and N-acyl glycines, are outlined in chapter 3 and these results are used to develop a two dimensional liquid chromatography tandem mass spectrometry analysis method. Multiple reaction monitoring was utilized as the detection mode due to the enhanced limit of detection obtained from mass filtering background and matrix components. The implementation of our developed analytical separation and detection approach for quantitation of these bioactive lipids in vertebrate samples and future implications of primary fatty acid amide and N-acyl glycine analysis are reviewed in chapter 4. In addition, the appendix summarizes a method for selective, sensitive multiple reaction monitoring detection of palmitoylethanolamine, an N-acylethanolamine with analgesic and anti-inflammatory properties.
Divito, E. (2013). Analysis Strategies for Bioactive, Polar Fatty Amides in Complex Samples (Doctoral dissertation, Duquesne University). Retrieved from https://dsc.duq.edu/etd/489