Environmental Science and Management (ESM)
Bayer School of Natural and Environmental Sciences
Fracking, Marcellus Shale, Pellicle, Salinivibrio costicola, Shale, Unconventional gas extraction
A euryhalic, gram-negative, facultatively anaerobic bacterium was isolated from an enrichment culture found in an impoundment used for storing Marcellus Shale waste water. Designated strain LP-1, its cells were non-spore-forming, motile, curved rods with a single polar flagellum. Phylogenetic analysis using 16S rRNA gene sequences revealed it is a £^-proteobacerium closely related (98%) to Salinivibrio costicola. Carbohydrates serve as energy sources both aerobically and anaerobically. It grew anaerobically on nitrate with hydrogen, acetate, pyruvate, or lactate but not with formate, and did not use arsenate, sulfate, or thiosulfate as electron acceptors. Strain LP-1 grew optimally at 37°C (range 4-65°C), 10.0% NaCl (range 0-20.0%), and a pH of 7.5 (range 4.5-10.5). It is capable of forming pellicles in liquid culture and Strontium and Barium-containing precipitates. These results suggest that the recycling impoundments contain unique microbiota that have adapted to living in a broad range of conditions.
Dugas, O. (2014). Isolation and Characterization of Salinivibrio sp. Strain LP-1 from an Impoundment Used for Marcellus Shale Waste Waters (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/506