Bayer School of Natural and Environmental Sciences
Peter A. Castric
Kyle W. Selcer
Biofilm, Gram-negative Bacteria, Microbial Physiology, Twitching Motility
The presence of Pseudomonas aeruginosa 1244 pili on the bacterial surface is dependent upon the growth environment. Quantitative western immunoblot analysis showed that an agar plate promoted surface expression of pili, whereas bacterial cells grown in liquid broth lacked similar appendages. Hyperexpression of the pilA gene increased piliation in broth cells but not to the extent of plate cells. Real-time RT-PCR indicated that the pilA gene has the potential to be regulated posttranscriptionally, regardless of the growth environment.
The P. aeruginosa 1244 pilin glycan and lipopolysaccharide O-antigen are synthesized via the same biosynthetic pathway. Rabbits immunized with LPS-free pili produced antibodies that recognized both pili and LPS. Serum from these animals was analyzed using ELISA assays, and the pilin glycan was found to be an important immunogenic epitope directing strong responses against both the glycan and O-antigen. This could prove useful in P. aeruginosa vaccine generation.
Furst, D. (2005). Pseudomonas aeruginosa 1244 Piliation: Environmental Signals and Regulation (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/561