Bayer School of Natural and Environmental Sciences
Richard P. Elinson
John S. Doctor
Phil G. Campbell
FGF, solid-phase, Xenopus
Cells become different in development due to induction, a ligand-mediated cell interaction. This type of interaction is an area of intense research for developmental biologists studying the effects of different growth factors (during development). The conventional approach to induction with Xenopus Laevis, African Clawed Frog, employs placing cells or tissues in a growth factor solution. This design reveals an enormous amount of information and insight into developmental processes, but it is not an accurate approach to simulate in vivo conditions. Here, I use a novel approach, termed solid-phase induction, as a more realistic and controllable approach. I utilize a glass surface coated with fibrin to act as a surface to adhere growth factors for Solid-phase presentation. This method will not only provide a more accurate representation of cell-cell inductions, but will also add an increased level of control in presenting growth factors. This approach will be used to answer developmental questions that involve spatially oriented induction and differential induction by different concentration gradients or specific patterns of a number of growth factors.
Maropis, P. (2006). Solid-Phase Induction of Xenopus Laevis Animal Caps by FGF-2: A Novel Developmental Approach (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/875