Bayer School of Natural and Environmental Sciences
Joseph R. McCormick
csp, gene expression, quantitative RT-PCR
The cold-shock response in Escherichia coli induces a set of 26 proteins that help ensure the efficiency of transcription, translation, and DNA replication at low temperatures. Some members of this group belong to the cold-shock protein (Csp) family. In Escherichia coli K12, there are 9 homologous csp genes named cspA-cspI. The Csp family in this strain is unusual because each gene has different mechanisms of induction and gene products. Various regulation studies have been performed on individual members of this family to help understand why so many homologues exist. No literature to date has ever looked at the mRNA expression patterns of all 9 csp genes simultaneously. This study used quantitative RT-PCR to establish normal csp mRNA expression patterns at different stages of growth. I found under normal conditions, the mRNA accumulation patterns of csp gene pairs (A/B, G/I, C/E, and F/H) were most similar to each other.
Mitchell, K. (2006). A Quantitative Approach Towards Establishing Normal csp mRNA Accumulation Patterns in Escherichia coli K12 (Master's thesis, Duquesne University). Retrieved from https://dsc.duq.edu/etd/938