Multidimensional liquid chromatography coupled with tandem mass spectrometry for identification of bioactive fatty acyl derivatives

Citation for published article

Voriconazole-associated periostitis: Pathophysiology, risk factors, clinical manifestations, diagnosis, and management

DOI

10.3389/fphys.2016.00608

Peer Reviewed

1

Document Type

Journal Article

Publication Date

1-1-2016

Publication Title

Frontiers in Physiology

Keywords

Bioactive lipids, Fatty acyls, Lipid-protein interactions, Multidimensional liquid chromatography, N-acyl ethanolamines, N-acyl glycines, Primary fatty acid amides

School

Bayer School of Natural and Environmental Sciences

Primary Author Department

Chemistry and Biochemistry

Abstract

© 2016 Divito, Kroniser and Cascio. Recognition of the contributions of lipids to cellular physiology, both as structural components of the membrane and as modulatory ligands for membrane proteins, has increased in recent years with the development of the biophysical and biochemical tools to examine these effects. Their modulatory roles in ion channels and transporters function have been extensively characterized, with the molecular mechanisms of these activities being the subject of intense scrutiny. The physiological significance of lipids in biochemistry is expanding as numerous fatty acyls are discovered to possess signaling properties. These bioactive lipids are often found in quantities of pmol/g of tissue and are co-extracted with numerous lipophilic molecules, making their detection and identification challenging. Common analytical methodologies involve chromatographic separation and mass spectrometric techniques; however, a single chromatographic step is typically ineffective due to the complexity of the biological samples. It is, therefore, essential to develop approaches that incorporate multiple dimensions of separation. Described in this manuscript are normal phase and reversed phase separation strategies for lipids that include detection of the bioactive primary fatty acid amides and N-acyl glycines via tandem mass spectrometry. Concerted utilization of these approaches are then used to separate and sensitively identify primary fatty acid amides extracted from homogenized tissue, using mouse brains as a test case.

Rights

© 2016 Divito, Kroniser and Cascio.

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