Recognition of novel objects and their location in rats with selective cholinergic lesion of the medial septum

DOI

10.1016/j.neulet.2011.11.019

Document Type

Journal Article

Publication Date

1-11-2012

Publication Title

Neuroscience Letters

Volume

506

Issue

2

First Page

261

Last Page

265

ISSN

3043940

Keywords

Cholinergic denervation, Hippocampus, Memory, Object recognition, Place recognition

Abstract

The importance of cholinergic neurons projecting from the medial septum (MS) of the basal forebrain to the hippocampus in memory function has been controversial. The aim of this study was to determine whether loss of cholinergic neurons in the MS disrupts object and/or object location recognition in male Sprague-Dawley rats. Animals received intraseptal injections of either vehicle, or the selective cholinergic immunotoxin 192 IgG-saporin (SAP). 14 days later, rats were tested for novel object recognition (NOR). Twenty-four hours later, these same rats were tested for object location recognition (OLR) (recognition of a familiar object moved to a novel location). Intraseptal injections of SAP produced an 86% decrease in choline acetyltransferase (ChAT) activity in the hippocampus, and a 31% decrease in ChAT activity in the frontal cortex. SAP lesion had no significant effect on NOR, but produced a significant impairment in OLR in these same rats. The results support a role for septo-hippocampal cholinergic projections in memory for the location of objects, but not for novel object recognition. © 2011 Elsevier Ireland Ltd.

Open Access

Green Accepted

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