Title

Secondary anchor substitutions in an HLA-A*0201-restricted T-cell epitope derived from Her-2/neu

DOI

10.1016/j.molimm.2006.02.027

Document Type

Journal Article

Publication Date

1-1-2007

Publication Title

Molecular Immunology

Volume

44

Issue

4

First Page

322

Last Page

331

ISSN

1615890

Keywords

Altered peptide ligand, Her-2/neu, HLA-A*0201, Molecular dynamics, Tumor vaccine

Abstract

We investigated analogues of GP2 (IISAVVGIL), an HLA-A*0201-restricted T-cell epitope derived from residues 654-662 in the tumor-associated antigen (TAA) Her-2/neu. One limiting factor of GP2 is its poor affinity for HLA-A*0201. Conformational analysis revealed the P5-P7 region in GP2 appears to be linked to the stability of P9 side chain interaction with the MHC molecule. To identify variants of GP2 with enhanced presentation to HLA-A*0201, we tested V6S, V6T, V6Q, G7P, G7F, T6F7, and Q6F7 for their capacity to stabilize cell surface HLA-A*0201 molecules. Of the mono-substituted variants, V6Q and G7F exhibited superior stabilization as compared to GP2. Molecular dynamics simulations suggest the improved binding can be attributed to concerted motions in the central and C-terminal regions of the peptide. These data support the notion that amino acids in HLA-A*0201 epitopes may be inter-dependent. Priming HLA-A*0201 transgenic mice with G7F-loaded syngeneic dendritic cells stimulated mouse T cells to produce a higher level of INFγ than mice immunized with GP2. © 2006 Elsevier Ltd. All rights reserved.

Open Access

Green Accepted

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