Secondary anchor substitutions in an HLA-A*0201-restricted T-cell epitope derived from Her-2/neu
Altered peptide ligand, Her-2/neu, HLA-A*0201, Molecular dynamics, Tumor vaccine
We investigated analogues of GP2 (IISAVVGIL), an HLA-A*0201-restricted T-cell epitope derived from residues 654-662 in the tumor-associated antigen (TAA) Her-2/neu. One limiting factor of GP2 is its poor affinity for HLA-A*0201. Conformational analysis revealed the P5-P7 region in GP2 appears to be linked to the stability of P9 side chain interaction with the MHC molecule. To identify variants of GP2 with enhanced presentation to HLA-A*0201, we tested V6S, V6T, V6Q, G7P, G7F, T6F7, and Q6F7 for their capacity to stabilize cell surface HLA-A*0201 molecules. Of the mono-substituted variants, V6Q and G7F exhibited superior stabilization as compared to GP2. Molecular dynamics simulations suggest the improved binding can be attributed to concerted motions in the central and C-terminal regions of the peptide. These data support the notion that amino acids in HLA-A*0201 epitopes may be inter-dependent. Priming HLA-A*0201 transgenic mice with G7F-loaded syngeneic dendritic cells stimulated mouse T cells to produce a higher level of INFγ than mice immunized with GP2. © 2006 Elsevier Ltd. All rights reserved.
Joseph, M., Mitchell, M., Evanseck, J., Kovacs, J., Jia, L., Shen, H., & Meng, W. (2007). Secondary anchor substitutions in an HLA-A*0201-restricted T-cell epitope derived from Her-2/neu. Molecular Immunology, 44 (4), 322-331. https://doi.org/10.1016/j.molimm.2006.02.027