Defense Date

12-10-2003

Graduation Date

Fall 2003

Availability

Immediate Access

Submission Type

thesis

Degree Name

MS

Department

Chemistry and Biochemistry

Committee Chair

Partha Basu

Committee Member

John F. Stolz

Committee Member

Mitchell E. Johnson

Keywords

arsenic, bacterial transformation, molybdenum, oxo-transfer

Abstract

The HPLC-UV-Vis method with 7.5 mM sulfuric acid as a mobile phase and 195 nm wavelength detection was used for the separation and quantification of As(III) and As(V) in bacterial media used for growing of Clostridium sp. Strain OhILAs bacteria. In this method, the arsenic species were eluted in order As(V) > As(III), and the retention time were not affected by bacteria media matrix. It has been shown that the shape of the curve of increasing of the As(III) concentration is similar to that of bacteria growing. The main disadvantage of the present method is its failure to separate As(III) ions and lactate. It has been proven that the HPLC method, even it has limitations, can be used for the determination of the concentration of As(V) and As(III) ions in bacteria medium.

A several new molybdenum (VI) complexes ([LMoO(p-OC6H4OEt)2](CB11H6Br6), [LMoO(p-OC6H4CN)2](CB11H6Br6), and LMoO2(p-OC6H4OEt) were synthesized and characterized by variety of spectroscopic techniques. For the first time, the intermediates of the oxygen-atom transfer reaction with participation of arsenic compounds ([LMo(OAsPh3)(p-OC6H4OEt)2](CB11H6Br6), [LMo(OAsPh3OH) (p-OC6H4CN)2](CB11H6Br6), and [LMo(OAsO(OH))(p-OC6H4CN)2](CB11H6Br6)) were detected by ESI MS method.

Format

PDF

Language

English

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