Defense Date
6-22-2010
Graduation Date
Fall 2010
Availability
Immediate Access
Submission Type
dissertation
Degree Name
PhD
Department
Pharmacology
School
School of Pharmacy
Committee Chair
Paula Witt-Enderby
Committee Member
Vicki Davis
Committee Member
Jane Cavanaugh
Committee Member
John Pollock
Committee Member
Rehana Leak
Keywords
Beta-arrestin, Desensitization, Melatonin receptors, Mesenchymal stem cells, Mutations, Osteoblasts
Abstract
Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone.
Format
Language
English
Recommended Citation
Sethi, S. (2010). Effect of Melatonin on Differentiation of Human Mesenchymal Stem Cells and a Study on C-Terminal Domains of MT1 and MT2 Melatonin Receptors (Doctoral dissertation, Duquesne University). Retrieved from https://dsc.duq.edu/etd/1173