Xue Chen

Defense Date

7-15-2015

Graduation Date

2015

Availability

Immediate Access

Submission Type

thesis

Degree Name

MS

Department

Environmental Science and Management (ESM)

Committee Chair

John Stolz

Committee Member

Nancy Trun

Committee Member

Partha Basu

Keywords

Arsenic, Bacteria, Genome

Abstract

Although arsenic is toxic to most living cells, many microbes live in environments with high concentrations of arsenic. In addition to being resistant to arsenic, some actively couple arsenic oxidation/reduction reactions to respiration gaining energy in the process. Following a current review of the literature, the genomes of the three such bacteria, one arsenite oxidizer, Alkalilimnicola ehrlichii MLHE-1, and two arsenate reducers, Alkaliphilus oremlandii OhILAs, and Bacillus selenitireducens MLS-10 are presented. The genome of Alkalilimnicola ehrlichii MLHE-1 consists of 3,275,944 bp with a 67.53% GC content; the genome of Alkaliphilus oremlandii OhILAs consists of 3,123,558 bp with a 36.26% GC content; and the genome of Bacillus selenitireducens MLS-10 consists of 3,592,478 bp with a 48.46% GC content. The arsenic genes in A. oremlandii were clustered in an "arsenic island", while the genes for arsenic resistance (i.e., ars) and respiration (arr, arx) in Al. ehrlichii and B. selenitireducens were not.

Format

PDF

Language

English

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