Defense Date

11-18-2013

Graduation Date

Spring 2013

Availability

Immediate Access

Submission Type

thesis

Degree Name

MS

Department

Environmental Science and Management (ESM)

Committee Chair

John F Stolz

Committee Member

Peter Castric

Committee Member

Brady Porter

Committee Member

Stanley Kabala

Keywords

Biofilms, Environmental microbiology, Flowback water, Geomicrobiology, Microbiology, Oil and Gas

Abstract

Flowback water generated during unconventional natural gas extraction in the Marcellus Shale contains elevated levels of total dissolved solids (TDS). Microbial growth is stimulated in these fluids during storage in open impoundments. The current study was conducted to isolate a strain from flowback water enrichment cultures and characterize it. An isolate was obtained by plate streaking and serial dilution methods and maintained on a nutrient broth with high TDS. Phylogenetic analysis using the 16S rRNA sequence revealed that bacterium was most closely related to Bacillus firmus<<<<<

Format

PDF

Language

English

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