Integrator Complex Subunit 3 Knockdown Has Minimal Effect on Lytic Herpes Simplex Virus Type-1 Infection in Fibroblast Cells
DOI
10.17912/micropub.biology.001171
Document Type
Journal Article
Publication Date
1-1-2024
Publication Title
microPublication biology
Volume
2024
Abstract
Proteomic analysis of viral and cellular proteins that copurify with the herpes simplex virus type-1 (HSV-1) genome revealed that the cellular Integrator complex associates with viral DNA throughout infection. The Integrator complex plays a key role in the regulation of transcription of cellular coding and non-coding RNAs. We therefore predicted that it may regulate transcription of viral genes. Here, we demonstrate that knockdown of the Integrator complex subunit, Ints3, has minimal effect on HSV-1 infection. Despite reducing viral yield during low multiplicity infection, Ints3 knockdown had no effect on viral DNA replication, mRNA expression, or yield during high multiplicity infection.
Open Access
38817634 (pubmed); PMC11137619 (pmc); 10.17912/micropub.biology.001171 (doi)
Preprint
Repository Citation
Heath, J. R., Fromuth, D. P., & Dembowski, J. A. (2024). Integrator Complex Subunit 3 Knockdown Has Minimal Effect on Lytic Herpes Simplex Virus Type-1 Infection in Fibroblast Cells. microPublication biology, 2024. https://doi.org/10.17912/micropub.biology.001171