Cellular Pharmacokinetic Model-Based Analysis of Genistein, Glyceollin, and MK-571 Effects on 5 (and 6)-Carboxy-2?,7?-Dichloroflourescein Disposition in Caco-2 Cells

DOI

10.1016/j.xphs.2017.12.004

Authors

Callie Drennen, Duquesne University
Erin Gorse, Duquesne University
Robert E. Stratford, Duquesne University

Document Type

Journal Article

Publication Date

4-1-2018

Publication Title

Journal of Pharmaceutical Sciences

Volume

107

Issue

4

First Page

1194

Last Page

1203

ISSN

223549

Keywords

active transport, Caco-2 cells, drug transport, efflux pumps, intestinal secretion/transport, membrane transport, pharmacokinetics, transcellular transport, transporters

Abstract

Pharmacokinetic modeling was used to describe 5 (and 6)-carboxy-2?,7?-dichloroflourescein (CDF) disposition in Caco-2 cells following CDF or CDFDA (CDF diacetate) dosing. CDF transcellular flux was modeled by simple passive diffusion. CDFDA dosing models were based on simultaneous fitting of CDF levels in apical, basolateral, and intracellular compartments. Predicted CDF efflux was 50% higher across the apical versus the basolateral membrane. This difference was similar following apical and basolateral CDFDA dosing, despite intracellular levels being 3-fold higher following basolateral dosing, thus supporting nonsaturable CDF efflux kinetics. A 3-compartment catenary model with intracellular CDFDA hydrolysis described CDF disposition. This model predicted that apical CDF efflux was not altered in the presence of MK-571, and that basolateral membrane clearance was enhanced to account for reduced intracellular CDF in the presence of this multidrug resistance-associated protein (MRP) inhibitor. Similar effects were predicted for glyceollin, while genistein exposure had no predicted effects on CDF efflux. These modulator effects are discussed in the context of model predicted intracellular CDF concentrations relative to reports of CDF affinity (measured by Km) for MRP2 and MRP3. This model-based analysis confirms the complexity of efflux kinetics and suggests that other transporters may have contributed to CDF efflux.

Open Access

Green Accepted

Preprint

Link to Author Manuscript

Repository Citation

Drennen, C., Gorse, E., & Stratford, R. (2018). Cellular Pharmacokinetic Model-Based Analysis of Genistein, Glyceollin, and MK-571 Effects on 5 (and 6)-Carboxy-2?,7?-Dichloroflourescein Disposition in Caco-2 Cells. Journal of Pharmaceutical Sciences, 107 (4), 1194-1203. https://doi.org/10.1016/j.xphs.2017.12.004