Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model
DOI
10.1016/j.clim.2015.04.019
Document Type
Journal Article
Publication Date
9-1-2015
Publication Title
Clinical Immunology
Volume
160
Issue
1
First Page
59
Last Page
70
ISSN
15216616
Keywords
Cyclooxygenase-2, Fluorescence, Imaging, Inflammation, Macrophage
Abstract
Targeting macrophages for therapeutic and diagnostic purposes is an attractive approach applicable to multiple diseases. Here, we present a theranostic nanoemulsion platform for simultaneous delivery of an anti-inflammatory drug (celecoxib) to macrophages and monitoring of macrophage migration patterns by optical imaging, as measurement of changes in inflammation. The anti-inflammatory effect of the theranostic nanoemulsions was evaluated in a mouse inflammation model induced with complete Freund's adjuvant (CFA). Nanoemulsions showed greater accumulation in the inflamed vs. control paw, with histology confirming their specific localization in CD68 positive macrophages expressing cyclooxygenase-2 (COX-2) compared to neutrophils. With a single dose administration of the celecoxib-loaded theranostic, we observed a reduction in fluorescence in the paw with time, corresponding to a reduction in macrophage infiltration. Our data strongly suggest that delivery of select agents to infiltrating macrophages can potentially lead to new treatments of inflammatory diseases where macrophage behavior changes are monitored in vivo.
Open Access
Green Accepted
Preprint
Repository Citation
Patel, S., Beaino, W., Anderson, C., & Janjic, J. (2015). Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model. Clinical Immunology, 160 (1), 59-70. https://doi.org/10.1016/j.clim.2015.04.019
