Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2

DOI

10.1074/jbc.M114.555177

Authors

Courtney E. Sparacino-Watkins, Allergy
Jesús Tejero, Allergy
Bin Sun, Allergy
Marc C. Gauthier, Allergy
John Thomas, Duquesne University
Venkata Ragireddy, Allergy
Bonnie A. Merchan, Allergy
Jun Wang, Allergy
Ivan Azarov, Allergy
Partha Basu, Duquesne University
Mark T. Gladwin, Allergy

Document Type

Journal Article

Publication Date

4-11-2014

Publication Title

Journal of Biological Chemistry

Volume

289

Issue

15

First Page

10345

Last Page

10358

ISSN

219258

Abstract

Background: Nitrite reduction pathways are critical for biological NO production under hypoxia. Results: The mitochondrial enzyme mARC reduces nitrite to NO using cytochrome b5 as electron donor. Conclusion:mARCforms an electron transfer chain withNADH,cytochrome b5, and cytochrome b5 reductase to reduce nitrite to NO. Significance: mARC proteins may constitute a new pathway for hypoxic NO production in vivo. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.

Open Access

Green Final

Repository Citation

Sparacino-Watkins, C., Tejero, J., Sun, B., Gauthier, M., Thomas, J., Ragireddy, V., Merchan, B., Wang, J., Azarov, I., Basu, P., & Gladwin, M. (2014). Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2. Journal of Biological Chemistry, 289 (15), 10345-10358. https://doi.org/10.1074/jbc.M114.555177