Defense Date

3-17-2010

Graduation Date

2010

Availability

Immediate Access

Submission Type

thesis

Degree Name

MS

Department

Medicinal Chemistry

Committee Chair

Patrick Flaherty

Committee Member

Aleem Gangjee

Committee Member

Marc Harrold

Committee Member

David Lapinsky

Committee Member

Jane Cavanaugh

Keywords

Benzimidazoles, Diphenylamine, Flavones, MEK-5, Ortho-Carboxyamide, Tubulysin

Abstract

CDK-5 is associated with hyperphosphorylation of the microtubule-associated protein tau, formation of neurofibrillary tangles, and possibly an acceleration of the neurodegenerative progression of Alzheimer's disease. C6-O linked benzimidazoles and C-4 benzamide and phenylacetamide benzimidazoles based on (R)-Roscovitine, a non-selective inhibitor of CDK-5 were designed and synthesized.

MEK-5, a member of the MAPK family, phosphorylates ERK-5 permitting cells to survive oxidative stress. MEK-5 is upregulated in tumor cells and activated by mitogens; inhibition of this enzyme is a potential anti-cancer strategy. Scaffolds from the following classes--benzimidazole, diphenylamine, flavones, and ortho-carboxyamide were examined for their capacity to be developed into potent and selective MEK-5 inhibitors.

Tubulysin is a natural product that disrupts microtubule dynamics, blocks mitosis, and induces cell death. It has been examined as a potential anti-cancer agent in hollow fiber assays. Simple and reliable procedures were developed for the gram-scale synthesis of Mep-Ile dipeptide and Tup fragments of tubulysin.

Format

PDF

Language

English

Share

COinS