Synthesis and biological activity of 5-chloro-N4-substituted phenyl-9H-pyrimido[4,5-b]indole-2,4-diamines as vascular endothelial growth factor receptor-2 inhibitors and antiangiogenic agents

DOI

10.1016/j.bmc.2013.01.040

Document Type

Journal Article

Publication Date

4-1-2013

Publication Title

Bioorganic and Medicinal Chemistry

Volume

21

Issue

7

First Page

1857

Last Page

1864

ISSN

9680896

Keywords

Cytotoxicity, Pyrimido[4, 5-b]indol synthesis, Receptor tryosine kinase inhibitors, VEGFR-2 inhibitors

Abstract

Inhibition of receptor tyrosine kinase (RTK) signaling pathways is an important area for the development of novel anticancer agents. Numerous multikinase inhibitors (MKIs) have been recently approved for the treatment of cancer. Vascular endothelial growth factor receptor-2 (VEGFR-2) is the principal mediator of tumor angiogenesis. In an effort to develop ATP-competitive VEGFR-2 selective inhibitors the 5-chloro-N4-substituted phenyl-9H-pyrimido[4,5-b]indole-2,4-diamine scaffold was designed. The synthesis of the target compounds involved N-(4,5-dichloro-9H-pyrimido[4,5-b]indol-2-yl)- 2,2-dimethylpropanamide) as a common intermediate. A nucleophilic displacement of the 4-chloro group of the common intermediate by appropriately substituted anilines afforded the target compounds. Biological evaluation indicated that compound 5 is a potent and selective VEGFR-2 inhibitor comparable to sunitinib and semaxinib. © 2013 Elsevier Ltd. All rights reserved.

Open Access

Green Accepted

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